Jaime Grutzendler MD
Associate Professor of Neurology and of Neurobiology; Director, Center for Experimental Neuroimaging
Neuro-glio-vascular interactions, cerebral blood flow and metabolism blood-brain barrier, two-photon microscopy, myelin imaging, synaptic plasticity, development and pathology of the brain microvasculature, Neuroinflammationin, neurodegenerative diseases and stroke
The dynamics and logic of neuro-glio-vascular interactions
in health and disease
The overall interest of our laboratory centers around cells in the so called "neuro-glio-vascular unit" (neurons, endothelium, astrocytes, pericytes, microglia and oligodendrocytes). Our goal is to learn about the dynamic properties of these cells and how cell-cell interactions are disrupted in brain injury, vascular and neurodegenerative pathologies.
We combine high-resolution cellular imaging in vivo and fixed tissues of single cells and small clusters of interacting cells, with novel optical sensors of cellular physiology, optogenetic tools and genetic manipulations using viral vectors, in utero electroporation and genome editing techniques.
Extensive Research Description
- IN VIVO IMAGING:The majority of projects in the laboratory are imaging-oriented. We use a variety of techniques including transcranial in vivo two photon microscopy, high-resolution confocal, spectral confocal reflectance microscopy (SCoRe) and electron microscopy in combination with differential fluorescent cell labeling and genetic/pharmacological manipulations. We aim at developeing novel imaging strategies and methods to better understand the pathophysiology of a variety of neurological disorders.
- MICROVASCULAR PLASTICITY: microvascular recanalization and angioplasticity after cerebral embolic occlusion. We have recently described a new mechanism of microvascular plasticity which leads to vessel recanalization (Angiophagy). We are investigating the molecular control and physiological relevance of this novel mechanism.
- "NEURO-GLIO-VASCULAR UNIT" FUNCTION AND PATHOLOGY: Understanding the postnatal development and pathology of neuro-glio-vascualr interactions, neurovascular coupling, tissue oxygenation. Astrocyte physiology.
- AXON AND SYNAPSE DEGENERATION/REGENERATION: Wa have recently developed a method for imaging myelinated axons in vivo (Spectral confocal reflectance microscopy SCoRe), which we are currently using to explore questions related to myelin's role in degeneration and regeneration.
- ALZHEIMER"S DISEAESE: understanding the dynamics of neurodegenerative conditions such as Alzheimer's disease. We study the interactions between microglia, neurons and amyloid plaques to understand the sequence of events leading to neural dysfunction in AD.