Diarrhea; Electrophysiology; Gastric Acid; Gastroenterology; Physiology
Our primary focus is on real time monitoring of ion transport mechanisms in epithelia, using high-resolution microscopy. One of the crucial problems facing all epithelial cells is the control of the intracellular milieu. In an effort to maintain ionic homeostasis, epithelial cells have devised a variety of ion channels, transport proteins, and carriers to regulate and maintain the intracellular ionic concentrations. To further understand how ions are transported in epithelial cells, we have developed a variety of optical techniques to continuously monitor intracellular ionic concentrations in real time. Using high-resolution video or confocal microscopy we are presently able to monitor intracellular Ca2+, Na+, K+, Cl-, pH, and membrane potential. We are currently investigating various aspects of renal tubule and gastric tissue ion transport in intact renal tubules and gastric glands. We also are characterizing the secretory and reabsorptive properties of the colonic crypt, with a special focus on diarrheal disease.
Specialized Terms: Gastric Acid Secretion; Colonic Fluid Transport; Renal Physiology; Intracellular Ion Activity Measurements; Calcium Sensing Receptor; Diarrheal disease; electrophysiology; CFTR; AMPKinase volume regulation
Extensive Research Description
We are focused on 3 main areas; gastric ion transport in health and disease, intestinal ion transport in health and disease, and renal physiology. For gastric ion transport we are focusing on means to modulate acid secretion and reverse erosion. We use a combination of natural products and biochemical modifications to treat acid disease at the cellular level. For studies of intestinal ion transport, we are focusing on how the calcium sensing receptor(CaSR) can modulate fluid transport during toxin induced diarrhea. In addition we are investigating means to prevent ischemic injury by activating ionic pathways using the AMPKinase. Finally we are trying to prevent IBD/IBS associated epithelial injury in the gut by Casr activation . For the renal studies we are interested in acid base homeostasis along the nephron, and are now investigating the role of the CaSR in modulating fluid secretion and renal stone formation.
Effect of NSAIDs on Na+/H+ exchanger activity in Rat colonic crypts
Roginiel AC, Kohut DL, Kaur S, Saleh AM, Weber T, Geibel P, Singh H, and Geibel JP. Effect of NSAIDs on Na+/H+ exchanger activity in Rat colonic crypts. American journal of physiology Cell physiology 2013
Gastric acid, calcium absorption, and their impact on bone health.
Kopic S, and Geibel JP. Gastric acid, calcium absorption, and their impact on bone health. Physiological reviews 93: 189-268, 2013.
The calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule
Capasso G, Geibel PJ, Damiano S, Jaeger P, Richards WG, and Geibel JP. The calcium sensing receptor modulates fluid reabsorption and acid secretion in the proximal tubule. Kidney international 2013.
Vacuolar-type H+-ATPase-mediated proton transport in the rat parietal cell.
Kopic S, Wagner ME, Griessenauer C, Socrates T, Ritter M, and Geibel JP. Vacuolar-type H+-ATPase-mediated proton transport in the rat parietal cell. Pflugers Archive : European journal of physiology 463: 419-427, 2012.
Functional role of NHE4 as a pH regulator in rat and human colonic crypts
Arena EA, Longo WE, Roberts KE, Geibel P, Nateqi J, Brandstetter M, and Geibel JP. Functional role of NHE4 as a pH regulator in rat and human colonic crypts. American journal of physiology Cell physiology 302: C412-418, 2012
Zinc salts provide a novel, prolonged and rapid inhibition of gastric acid secretion
Kirchhoff P, Socrates T, Sidani S, Duffy A, Breidthardt T, Grob C, Viehl CT, Beglinger C, Oertli D, and Geibel JP. Zinc salts provide a novel, prolonged and rapid inhibition of gastric acid secretion. The American journal of gastroenterology 106: 62-70, 2011.